Experienced my first disconnect for unknown reasons in iSpy. Important to note that while the RTSP feed was offline, the normal feed through the WyzeApp was fine, indicating that the camera was up and running (V2).
Further verified the RTSP feed was not available and it was not an issue with iSpy by trying to connect directly with VLC.
The connection was re-established by simply regenerating the RTSP URL under Adv settings in the WyzeApp. (Note here that RTSP was not switched off and back on as I believe others have reported. The only action was to choose to regenerate the URL)